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- SP PuraBead® Edge
SP PuraBead® Edge
SP PuraBead® Edge is one of the next generation of IEX resins from Astrea Bioseparations. The high performance PuraBead® Edge resin bead is ideal for high resolution polishing applications with short residence times and low back pressure. PuraBead® Edge utilizes the same high performance ion exchange ligands as our P6HF family of ion exchangers, providing proven performance and increasing productivity over other small chromatography beads.
Features and Benefits
- Improved resolution
- High binding capacity, for example 95 mg/mL for Lysozyme
- High flow rates of up to 300 cm/h to quickly process large volumes
- Robust, long life adsorbent
- Sanitizable with NaOH allowing for multiple cycles
- Optimal balance of flow rate, binding capacity and resolution to maximize productivity
Property | Value |
---|---|
Ligand | Sulphopropyl group |
Type of Ion Exchanger | Strong cation |
Total Ionic Capacity | 97 to 163 µmol/g settled gel |
Mean Particle Size (µm) | 65 ± 10 µm |
Matrix | PuraBead® P60HF (Highly cross-linked 6% near-monodisperse agarose) |
Binding Capacity | Up to 95 mg/mL of resin (Lysozyme, when loaded to 10% breakthrough) |
Recommended Packing Conditions | Pack at a constant pressure of up to 3.5 bar |
Recommended Packing Solution | 1 M NaCl solution |
Recommended Operational Flow Rates | Up to 400 cm/h |
Operating pH | pH 4.0 to pH 14.0 |
Chemical Stablity | All commonly used aqueous buffers and co-solvents |
Cleaning/Sanitization | 0.5 to 1.0 M NaOH |
Sterilization | Autoclavable in 0.1 M NaCl solution at 121 °C for 30 minutes |
Storage | 2–30 °C, 20% ethanol, 0.2 M sodium acetate |
Column Packing Services
Need this resin packed for your project? Our column packing services are designed to deliver the speed and precision you need. From quick turnaround times to personalized support, we pack nearly any resin to ensure high-quality results every time.
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