Plasmid DNA
Plasmid DNA purification is essential in molecular biology, supporting genetic engineering, gene therapy, and protein production. Circular DNA structures, or plasmids, contain specific genes, and their purity is critical for safe and successful downstream applications. Techniques like column chromatography or precipitation remove contaminants, ensuring highly concentrated and pure plasmid DNA. This purity is crucial for accurate cloning, transfection, gene expression studies, and the development of gene-based therapies. High-quality plasmid DNA is necessary for regulatory compliance and research reproducibility, highlighting the fundamental role of plasmid DNA purification in modern biotechnology.
Our Solutions
pDNAHERO® polish, a novel HIC adsorbent, offers an efficient orthogonal chromatography step for contaminant removal after AEX primary capture or post linearization.
Even at fast flow rates, binding capacity remains high, and delta pressure across the device remains low. Performance is conserved as plasmid size increases, ensuring consistent yields as projects change.
Hydrophobic interaction chromatography (HIC) is an established method for separating DNA isoforms, and RNA by exploiting the different nucleic acid impurities.
Capacity and flow rates no longer need to be compromised when purifying larger DNA sequences, namely those required for cell & gene therapy applications.
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Flexible and scalable, DEAE PuraBead® P6HF provides the selectivity required for pDNA capture from the bench to the factory floor.
The variable selectivity provided by DEAE weak anion exchange chromatography resins preferentially capture pDNA while allowing contaminants, such as other nucleic acids and HCPs, to pass through the column.
- High capacity DEAE resin
- Can be used in a range of conditions to select for capture of pDNA only
- Large bead sizes and fast flow rates boost productivity
- Available in slurry and columns across a range of sizes to meet production needs
- Low shear forces reduce risk of damaging scDNA
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Ensure your sample contains only supercoiled pDNA with Hexyl PuraBead® P6HF.
Product degradation due to mechanical sheer stresses can contaminate supercoiled pDNA with open circular form which can be less effective.
- Removal of ocDNA increases the biological activity of pDNA product
- Open coiled ocDNA is not bound by Hexyl PuraBead® P6HF
- scDNA is captured and can be eluted
- Demonstrated scalability from bench scale to manufacturing
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