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SP PuraBead® Edge
SP PuraBead® Edge

SP PuraBead® Edge

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High resolution strong cation exchange chromatography resin on the agarose PuraBead® platform.

SP PuraBead® Edge is one of the next generation of IEX resins from Astrea Bioseparations. The high performance PuraBead® Edge resin bead is ideal for high resolution polishing applications with short residence times and low back pressure. PuraBead® Edge utilizes the same high performance ion exchange ligands as our P6HF family of ion exchangers, providing proven performance and increasing productivity over other small chromatography beads.

Features and Benefits
  • Improved resolution
  • High binding capacity, for example 95 mg/mL for Lysozyme
  • High flow rates of up to 300 cm/h to quickly process large volumes
  • Robust, long life adsorbent
  • Sanitizable with NaOH allowing for multiple cycles
  • Optimal balance of flow rate, binding capacity and resolution to maximize productivity
Specifications
PropertyValue
LigandSulphopropyl group
Type of Ion ExchangerStrong cation
Total Ionic Capacity97 to 163 µmol/g settled gel
Mean Particle Size (µm)65 ± 10 µm
MatrixPuraBead® P60HF
(Highly cross-linked 6% near-monodisperse agarose)
Binding CapacityUp to 95 mg/mL of resin (Lysozyme, when loaded to 10% breakthrough)
Recommended Packing ConditionsPack at a constant pressure of up to 3.5 bar
Recommended Packing Solution1 M NaCl solution
Recommended Operational Flow RatesUp to 400 cm/h
Operating pHpH 4.0 to pH 14.0
Chemical StablityAll commonly used aqueous buffers and co-solvents
Cleaning/Sanitization0.5 to 1.0 M NaOH
SterilizationAutoclavable in 0.1 M NaCl solution at 121 °C for 30 minutes
Storage2–30 °C, 20% ethanol, 0.2 M sodium acetate
 
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