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Enzymes
Enzymes, the biological catalysts essential for accelerating biochemical reactions, are indispensable across all living organisms and have a vast range of commercial applications—from research and diagnostics, food and biochemicals production to therapeutics. Ensuring the purity and activity of enzymes is critical, particularly when they are used in sensitive processes. Enzymes often require extensive purification to remove unwanted enzyme activities. The presence of enzymes such as proteases and esterases in feedstocks can be problematic and their prompt removal post-fermentation or post-cell culture is important to minimize the formation of product-related impurities. Astrea Bioseparations produces a range of products optimized for enzyme capture and purification.
Our Solutions
Purification and removal of serine proteases with p-Aminobenzamidine A6XL
Our p-Aminobenzamidine A6XL resin is engineered to bind to the active sites of a wide array of serine proteases, including trypsin, thrombin, kallikrein, and chymotrypsin, as well as certain sulfhydryl proteases and esterases such as papain and acetylcholinesterase. This selective binding capability makes p-Aminobenzamidine A6XL an ideal solution for both the capture and purification of trypsin-like proteases and the removal of undesired proteolytic activities in biological preparations.
Capture and purification of alkaline phosphatase with Mimetic Blue® AP HL P6HF
Alkaline phosphatase plays a pivotal role in numerous analytical and diagnostic procedures, particularly in immunoassays where the enzyme's purity and specific activity are crucial for achieving optimal assay sensitivity. Our Mimetic Blue® AP HL P6HF features a highly specific affinity ligand with a phosphonic acid group, anchored to our robust, fully alkali-stable PuraBead® 6HF base matrix. This unique configuration enables selective binding of alkaline phosphatase from a broad spectrum of sources, including human and animal tissue extracts and recombinant alkaline phosphatases produced by fermentation or mammalian cell cultures. A high ligand loading ensures superior binding capacities for alkaline phosphatase and maximizes process productivity.
Purification of nucleic acid modifying enzymes with PE PuraBead®
Our PE PuraBead® represents a modern, innovative alternative to traditional phosphocellulose materials, designed specifically for the purification of phosphate-binding proteins such as DNA and RNA modifying enzymes, restriction endonucleases, polymerases, and ligases. This resin includes a phosphoethanolamine group attached to our rugged, fully alkali-stable PuraBead® 6HF base matrix, enabling effective cleaning and sanitization with 0.5M or 1M NaOH. PE PuraBead® supports high flow rates, low back pressures, and long operational lifetimes, making it a highly reliable and efficient choice for achieving high purity nucleic acid modifying enzymes.
Capture and purification of cofactor binding enzymes with Mimetic Blue® SA resins
Originally designed for serum albumin purification, our Mimetic Blue® SA resins are equally adept at purifying enzymes requiring adenine nucleotide cofactors for catalytic activity, such as NAD(H), NADP(H), AMP, ADP, and ATP. The unique blue chromophoric component of the Mimetic Blue® SA ligand mimics the binding of adenine nucleotides to the active sites of key enzymes including dehydrogenases and kinases. These resins are not only highly robust but also support high flow rates, low back pressures, high purities and recoveries, and they can be cleaned and sanitized with NaOH, ensuring long operational lifetimes.
Enzyme purification by PuraBead® IEX
Ion Exchange (IEX) chromatography is a staple in the purification of a broad spectrum of enzyme activities. Astrea Bioseparations offers a comprehensive array of robust, fully alkali-stable ion exchange resins based on our free-flowing, near-monodisperse PuraBead® 6HF base matrix. These resins provide high binding capacities, long operational lifetimes, high flow rates, and low back pressures. With our pre-packed RoboColumns® and IEX Selection Kits, selecting the ideal PuraBead® ion-exchange resin and chromatography buffers for your application is straightforward, enabling the reproducible achievement of high yields of purified enzymes.
Enzyme purification by PuraBead® HIC
Our Hydrophobic Interaction Chromatography (HIC) resins are a preferred choice for scientists seeking to optimize enzyme purification and impurity removal. We offer a diverse range of robust, fully alkali-stable hydrophobic interaction resins. These resins are constructed from our free-flowing, near-monodisperse PuraBead® 6HF base matrix, celebrated for its high binding capacities, long operational lifetimes, high flow rates, and low back pressures. The selection of the ideal PuraBead® HIC resin and corresponding chromatography buffers is streamlined with our pre-packed RoboColumns® and HIC Selection Kits, ensuring a seamless and efficient purification process. Our HIC resins are perfect for reproducibly achieving high yields of purified enzymes.
